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α ints6  (Novus Biologicals)


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    Structured Review

    Novus Biologicals α ints6
    a , Immunoblot detection of successful knock-down of INTS1, INTS3, <t>INTS6,</t> INTS7, and INTS11 before and after shRNA induction with Doxycyclin (Dox) at 1 µg/ml. GAPDH was used as loading control. b , Immunoblot of shControl before and after induction using the same INTS antibodies. c-f , Volcano plot comparing statistical significance and miRNA log 2 fold change between control and knock-down cells. Significantly regulated miRNAs are depicted in red. c , uninduced shControl compared to induced shControl. d , shINTS1 compared to induced shControl. e , shINTS3. f , shINTS7. g , Heat map of normalized miRNA expression from shControl, shINTS6, and shINTS11 (Z-score of normalized read counts per row). Column and row orders were determined by unsupervised hierarchical clustering. h , Immunoblot detection of Drosha after siRNA knock-down in HeLa. i , Volcano plot comparing statistical significance and miRNA log 2 fold change between siControl and siDrosha knock-down HeLa cells. Significantly regulated miRNAs are depicted in red. Drosha-independent miRNAs are indicated. j , k , Relative miRNA expression levels in j , HeLa shControl, shINTS6, and shINTS11 cells, or k , HEK293T cells transfected with siControl, siINTS6, siINTS11, or siDrosha. MiRNAs were detected by specific TaqMan probes for the indicated miRNAs and relative miRNA levels were calculated against RNU43 expression and shControl/siControl using ΔΔct method. Mean ± SEM, n = 4. Drosha-independent miRNA examples are indicated in red.
    α Ints6, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+ints6/INTS6+Antibody/bio_rxiv__2021__09__21__461113-66-23-24
    Average 93 stars, based on 2 article reviews
    α ints6 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "The Integrator complex regulates microRNA abundance through RISC loading"

    Article Title: The Integrator complex regulates microRNA abundance through RISC loading

    Journal: bioRxiv

    doi: 10.1101/2021.09.21.461113

    a , Immunoblot detection of successful knock-down of INTS1, INTS3, INTS6, INTS7, and INTS11 before and after shRNA induction with Doxycyclin (Dox) at 1 µg/ml. GAPDH was used as loading control. b , Immunoblot of shControl before and after induction using the same INTS antibodies. c-f , Volcano plot comparing statistical significance and miRNA log 2 fold change between control and knock-down cells. Significantly regulated miRNAs are depicted in red. c , uninduced shControl compared to induced shControl. d , shINTS1 compared to induced shControl. e , shINTS3. f , shINTS7. g , Heat map of normalized miRNA expression from shControl, shINTS6, and shINTS11 (Z-score of normalized read counts per row). Column and row orders were determined by unsupervised hierarchical clustering. h , Immunoblot detection of Drosha after siRNA knock-down in HeLa. i , Volcano plot comparing statistical significance and miRNA log 2 fold change between siControl and siDrosha knock-down HeLa cells. Significantly regulated miRNAs are depicted in red. Drosha-independent miRNAs are indicated. j , k , Relative miRNA expression levels in j , HeLa shControl, shINTS6, and shINTS11 cells, or k , HEK293T cells transfected with siControl, siINTS6, siINTS11, or siDrosha. MiRNAs were detected by specific TaqMan probes for the indicated miRNAs and relative miRNA levels were calculated against RNU43 expression and shControl/siControl using ΔΔct method. Mean ± SEM, n = 4. Drosha-independent miRNA examples are indicated in red.
    Figure Legend Snippet: a , Immunoblot detection of successful knock-down of INTS1, INTS3, INTS6, INTS7, and INTS11 before and after shRNA induction with Doxycyclin (Dox) at 1 µg/ml. GAPDH was used as loading control. b , Immunoblot of shControl before and after induction using the same INTS antibodies. c-f , Volcano plot comparing statistical significance and miRNA log 2 fold change between control and knock-down cells. Significantly regulated miRNAs are depicted in red. c , uninduced shControl compared to induced shControl. d , shINTS1 compared to induced shControl. e , shINTS3. f , shINTS7. g , Heat map of normalized miRNA expression from shControl, shINTS6, and shINTS11 (Z-score of normalized read counts per row). Column and row orders were determined by unsupervised hierarchical clustering. h , Immunoblot detection of Drosha after siRNA knock-down in HeLa. i , Volcano plot comparing statistical significance and miRNA log 2 fold change between siControl and siDrosha knock-down HeLa cells. Significantly regulated miRNAs are depicted in red. Drosha-independent miRNAs are indicated. j , k , Relative miRNA expression levels in j , HeLa shControl, shINTS6, and shINTS11 cells, or k , HEK293T cells transfected with siControl, siINTS6, siINTS11, or siDrosha. MiRNAs were detected by specific TaqMan probes for the indicated miRNAs and relative miRNA levels were calculated against RNU43 expression and shControl/siControl using ΔΔct method. Mean ± SEM, n = 4. Drosha-independent miRNA examples are indicated in red.

    Techniques Used: Western Blot, Knockdown, shRNA, Control, Expressing, Transfection

    Related Articles

    Western Blot:

    Article Title: The Integrator complex regulates microRNA abundance through RISC loading
    Article Snippet: Whole-cell RIPA lysates were prepared in presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084).presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084). ... After transfer on nitrocellulose membranes, we detected our protein of interest using the following antibodies: α-INTS1 (Bethyl Laboratories, #A300-361A), α-INTS3 (Sigma Prestige, HPA074391), α-INTS6 (Novus Biologicals, NB10086990), α-INTS7 (Bethyl Laboratories, A300-271A), α-INTS11 (Sigma Prestige, HPA029025), α-GAPDH (Abcam, ab8245), α-Drosha (Abcam, ab12286), α-Dicer (Abcam, ab14601), α-DGCR8 (Abcam, ab90579), α-Ago1 (Cell Signaling, D84G10), α-Ago2 (Abcam, ab57113), α-Ago3 (Sigma-Aldrich, SAB4200112), α-Ago4 (Cell Signaling, D10F10), α-Lamin B1 (Proteintech, #66095-1-1g).

    Knockdown:

    Article Title: The Integrator complex regulates microRNA abundance through RISC loading
    Article Snippet: Whole-cell RIPA lysates were prepared in presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084).presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084). ... After transfer on nitrocellulose membranes, we detected our protein of interest using the following antibodies: α-INTS1 (Bethyl Laboratories, #A300-361A), α-INTS3 (Sigma Prestige, HPA074391), α-INTS6 (Novus Biologicals, NB10086990), α-INTS7 (Bethyl Laboratories, A300-271A), α-INTS11 (Sigma Prestige, HPA029025), α-GAPDH (Abcam, ab8245), α-Drosha (Abcam, ab12286), α-Dicer (Abcam, ab14601), α-DGCR8 (Abcam, ab90579), α-Ago1 (Cell Signaling, D84G10), α-Ago2 (Abcam, ab57113), α-Ago3 (Sigma-Aldrich, SAB4200112), α-Ago4 (Cell Signaling, D10F10), α-Lamin B1 (Proteintech, #66095-1-1g).

    shRNA:

    Article Title: The Integrator complex regulates microRNA abundance through RISC loading
    Article Snippet: Whole-cell RIPA lysates were prepared in presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084).presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084). ... After transfer on nitrocellulose membranes, we detected our protein of interest using the following antibodies: α-INTS1 (Bethyl Laboratories, #A300-361A), α-INTS3 (Sigma Prestige, HPA074391), α-INTS6 (Novus Biologicals, NB10086990), α-INTS7 (Bethyl Laboratories, A300-271A), α-INTS11 (Sigma Prestige, HPA029025), α-GAPDH (Abcam, ab8245), α-Drosha (Abcam, ab12286), α-Dicer (Abcam, ab14601), α-DGCR8 (Abcam, ab90579), α-Ago1 (Cell Signaling, D84G10), α-Ago2 (Abcam, ab57113), α-Ago3 (Sigma-Aldrich, SAB4200112), α-Ago4 (Cell Signaling, D10F10), α-Lamin B1 (Proteintech, #66095-1-1g).

    Control:

    Article Title: The Integrator complex regulates microRNA abundance through RISC loading
    Article Snippet: Whole-cell RIPA lysates were prepared in presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084).presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084). ... After transfer on nitrocellulose membranes, we detected our protein of interest using the following antibodies: α-INTS1 (Bethyl Laboratories, #A300-361A), α-INTS3 (Sigma Prestige, HPA074391), α-INTS6 (Novus Biologicals, NB10086990), α-INTS7 (Bethyl Laboratories, A300-271A), α-INTS11 (Sigma Prestige, HPA029025), α-GAPDH (Abcam, ab8245), α-Drosha (Abcam, ab12286), α-Dicer (Abcam, ab14601), α-DGCR8 (Abcam, ab90579), α-Ago1 (Cell Signaling, D84G10), α-Ago2 (Abcam, ab57113), α-Ago3 (Sigma-Aldrich, SAB4200112), α-Ago4 (Cell Signaling, D10F10), α-Lamin B1 (Proteintech, #66095-1-1g).

    Expressing:

    Article Title: The Integrator complex regulates microRNA abundance through RISC loading
    Article Snippet: Whole-cell RIPA lysates were prepared in presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084).presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084). ... After transfer on nitrocellulose membranes, we detected our protein of interest using the following antibodies: α-INTS1 (Bethyl Laboratories, #A300-361A), α-INTS3 (Sigma Prestige, HPA074391), α-INTS6 (Novus Biologicals, NB10086990), α-INTS7 (Bethyl Laboratories, A300-271A), α-INTS11 (Sigma Prestige, HPA029025), α-GAPDH (Abcam, ab8245), α-Drosha (Abcam, ab12286), α-Dicer (Abcam, ab14601), α-DGCR8 (Abcam, ab90579), α-Ago1 (Cell Signaling, D84G10), α-Ago2 (Abcam, ab57113), α-Ago3 (Sigma-Aldrich, SAB4200112), α-Ago4 (Cell Signaling, D10F10), α-Lamin B1 (Proteintech, #66095-1-1g).

    Transfection:

    Article Title: The Integrator complex regulates microRNA abundance through RISC loading
    Article Snippet: Whole-cell RIPA lysates were prepared in presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084).presence of Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, #1861282) and proteins were separated by 4-15% Criterion TGX Stain-Free precast polyacrylamide gels (Biorad, # 5678084). ... After transfer on nitrocellulose membranes, we detected our protein of interest using the following antibodies: α-INTS1 (Bethyl Laboratories, #A300-361A), α-INTS3 (Sigma Prestige, HPA074391), α-INTS6 (Novus Biologicals, NB10086990), α-INTS7 (Bethyl Laboratories, A300-271A), α-INTS11 (Sigma Prestige, HPA029025), α-GAPDH (Abcam, ab8245), α-Drosha (Abcam, ab12286), α-Dicer (Abcam, ab14601), α-DGCR8 (Abcam, ab90579), α-Ago1 (Cell Signaling, D84G10), α-Ago2 (Abcam, ab57113), α-Ago3 (Sigma-Aldrich, SAB4200112), α-Ago4 (Cell Signaling, D10F10), α-Lamin B1 (Proteintech, #66095-1-1g).



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    α ints6  (Novus Biologicals)
    93
    Novus Biologicals α ints6
    a , Immunoblot detection of successful knock-down of INTS1, INTS3, <t>INTS6,</t> INTS7, and INTS11 before and after shRNA induction with Doxycyclin (Dox) at 1 µg/ml. GAPDH was used as loading control. b , Immunoblot of shControl before and after induction using the same INTS antibodies. c-f , Volcano plot comparing statistical significance and miRNA log 2 fold change between control and knock-down cells. Significantly regulated miRNAs are depicted in red. c , uninduced shControl compared to induced shControl. d , shINTS1 compared to induced shControl. e , shINTS3. f , shINTS7. g , Heat map of normalized miRNA expression from shControl, shINTS6, and shINTS11 (Z-score of normalized read counts per row). Column and row orders were determined by unsupervised hierarchical clustering. h , Immunoblot detection of Drosha after siRNA knock-down in HeLa. i , Volcano plot comparing statistical significance and miRNA log 2 fold change between siControl and siDrosha knock-down HeLa cells. Significantly regulated miRNAs are depicted in red. Drosha-independent miRNAs are indicated. j , k , Relative miRNA expression levels in j , HeLa shControl, shINTS6, and shINTS11 cells, or k , HEK293T cells transfected with siControl, siINTS6, siINTS11, or siDrosha. MiRNAs were detected by specific TaqMan probes for the indicated miRNAs and relative miRNA levels were calculated against RNU43 expression and shControl/siControl using ΔΔct method. Mean ± SEM, n = 4. Drosha-independent miRNA examples are indicated in red.
    α Ints6, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+ints6/INTS6+Antibody/bio_rxiv__2021__09__21__461113-66-23-24
    Average 93 stars, based on 1 article reviews
    α ints6 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    a , Immunoblot detection of successful knock-down of INTS1, INTS3, INTS6, INTS7, and INTS11 before and after shRNA induction with Doxycyclin (Dox) at 1 µg/ml. GAPDH was used as loading control. b , Immunoblot of shControl before and after induction using the same INTS antibodies. c-f , Volcano plot comparing statistical significance and miRNA log 2 fold change between control and knock-down cells. Significantly regulated miRNAs are depicted in red. c , uninduced shControl compared to induced shControl. d , shINTS1 compared to induced shControl. e , shINTS3. f , shINTS7. g , Heat map of normalized miRNA expression from shControl, shINTS6, and shINTS11 (Z-score of normalized read counts per row). Column and row orders were determined by unsupervised hierarchical clustering. h , Immunoblot detection of Drosha after siRNA knock-down in HeLa. i , Volcano plot comparing statistical significance and miRNA log 2 fold change between siControl and siDrosha knock-down HeLa cells. Significantly regulated miRNAs are depicted in red. Drosha-independent miRNAs are indicated. j , k , Relative miRNA expression levels in j , HeLa shControl, shINTS6, and shINTS11 cells, or k , HEK293T cells transfected with siControl, siINTS6, siINTS11, or siDrosha. MiRNAs were detected by specific TaqMan probes for the indicated miRNAs and relative miRNA levels were calculated against RNU43 expression and shControl/siControl using ΔΔct method. Mean ± SEM, n = 4. Drosha-independent miRNA examples are indicated in red.

    Journal: bioRxiv

    Article Title: The Integrator complex regulates microRNA abundance through RISC loading

    doi: 10.1101/2021.09.21.461113

    Figure Lengend Snippet: a , Immunoblot detection of successful knock-down of INTS1, INTS3, INTS6, INTS7, and INTS11 before and after shRNA induction with Doxycyclin (Dox) at 1 µg/ml. GAPDH was used as loading control. b , Immunoblot of shControl before and after induction using the same INTS antibodies. c-f , Volcano plot comparing statistical significance and miRNA log 2 fold change between control and knock-down cells. Significantly regulated miRNAs are depicted in red. c , uninduced shControl compared to induced shControl. d , shINTS1 compared to induced shControl. e , shINTS3. f , shINTS7. g , Heat map of normalized miRNA expression from shControl, shINTS6, and shINTS11 (Z-score of normalized read counts per row). Column and row orders were determined by unsupervised hierarchical clustering. h , Immunoblot detection of Drosha after siRNA knock-down in HeLa. i , Volcano plot comparing statistical significance and miRNA log 2 fold change between siControl and siDrosha knock-down HeLa cells. Significantly regulated miRNAs are depicted in red. Drosha-independent miRNAs are indicated. j , k , Relative miRNA expression levels in j , HeLa shControl, shINTS6, and shINTS11 cells, or k , HEK293T cells transfected with siControl, siINTS6, siINTS11, or siDrosha. MiRNAs were detected by specific TaqMan probes for the indicated miRNAs and relative miRNA levels were calculated against RNU43 expression and shControl/siControl using ΔΔct method. Mean ± SEM, n = 4. Drosha-independent miRNA examples are indicated in red.

    Article Snippet: After transfer on nitrocellulose membranes, we detected our protein of interest using the following antibodies: α-INTS1 (Bethyl Laboratories, #A300-361A), α-INTS3 (Sigma Prestige, HPA074391), α-INTS6 (Novus Biologicals, NB10086990), α-INTS7 (Bethyl Laboratories, A300-271A), α-INTS11 (Sigma Prestige, HPA029025), α-GAPDH (Abcam, ab8245), α-Drosha (Abcam, ab12286), α-Dicer (Abcam, ab14601), α-DGCR8 (Abcam, ab90579), α-Ago1 (Cell Signaling, D84G10), α-Ago2 (Abcam, ab57113), α-Ago3 (Sigma-Aldrich, SAB4200112), α-Ago4 (Cell Signaling, D10F10), α-Lamin B1 (Proteintech, #66095-1-1g).

    Techniques: Western Blot, Knockdown, shRNA, Control, Expressing, Transfection